Reagents and Equipment for CodeLink™ Bioarray Assays

Reagents
Ambion MessageAmp™ II-Biotin Enhanced Single Round aRNA Amplification Kit (20 reactions), Catalog # AM1791 (or equivalent biotin incorporating amplification kit)
Absolute (100%) Ethanol (molecular biology grade or higher)
Streptavidin-Cy5 for Microarrays (AMI Cat# 28900224)
0.5% NEN Blocking reagent (PerkinElmer Cat. # FP1020)
Nuclease-free water
1M Tris-HCl, pH 7.6
5M NaCl
Tween-20
20X SSC (Ambion Cat. # 9763)
Isopropanol (to wash reagent container for TNT preparation)

General lab equipment and consumables
UV spectrophotometer
Pipettors (10-, 20-, 200-, and 100-ul)
Pipette tips: sterile, RNase-free and aerosol resistant
Vortex mixer
Microcentrifuge
Heat block or water bath (for 46ºC, 50-55ºC, 70ºC, 90ºC)
Air incubator (37ºC, 42ºC)
Refrigerated incubator (16ºC)
Agilent™ bioanalyzer (or equivalent)
Innova 4080 shaking incubator (or equivalent)
CodeLink™ bioarray compatible scanner, such as:
GenePix™ 4000 Array Scanner
GenePix™ Personal 4100
Agilent™ G2565 microarray scanners
arrayWoRx®e microarray scanner
ScanArray™ Express scanners
GeneTAC™ UC4 Array Scanner
428™ Array Scanner
Centrifuge, 4-15ºC (QIAGEN Corp, 81010 or similar) with appropriate centrifuge plate rotor, 2x96 (QIAGEN Corp. Cat. #  81031)
1.7ml microcentrifuge tubes, sterile and RNase-free
15ml and 50ml conical tubes, sterile and nuclease-free
Wide bore pipette tips, sterile, RNase-fee and aerosol-resistant
Thermal cycler
Powder-free gloves

Equipment and consumables (CodeLink™-specific)
CodeLink™ Expression Analysis software (AMI Cat# 310035)
CodeLInk™ Universal Shaker Kit, 12 slides (AMI Cat. # 310031)
CodeLink™ Parallel Processing Kit, 12 slides (AMI Cat. # 310010)
Microtiter plate lid, black (Corning Corp. Cat. # 3935)